The lymphocyte receptors human CD69 and rat NKR-P1A have been shown as sacharide binding proteins in 26 articles. In order to utilize their potential in antitumor therapy many high affinity saccharide ligands have been synthesized over the last 16 years.
Due to the scientific misconduct investigation and serious suspicion that Karel Bezouska, author of all these articles, had been engaged in fabrication of primary experimental data, we have decided to re-examine the interaction between multiple ligands and CD69 or NKR-P1A.
Using the NMR titration and isothermal titration calorimetry, we were unable to detect binding of tested ligands, such as N-acetylhexosamines and oligopeptides to both receptors, which is in the sharp contradiction to the previous 26 articles.
