Sugars and Post-Translational Modifications are critical biological markers that modulate the properties of Proteins. Our work studies the interplay of proteins, sugars and modifications in many contexts. This lecture will cover recent example(s) from emerging areas in our group in (a) biomolecule and glycoconjugate chemistry with an emphasis on new bond-forming processes compatible within vitroand in vivobiology and (b) use of these biomolecules and chemistries to determine fundamental mechanisms and to create modulators of carbohydrate– & protein– processing events.
(a) Synthetic Biology’s development at the start of this century may be compared with Synthetic Organic Chemistry’s expansion at the start of the last; after decades of isolation, identification, analysis and functional confirmation the future logical and free-ranging redesign of biomacromolecules offers tantalizing opportunities. New methods are required: despite 80-years-worth of non-specific, chemical modification of proteins, precise methods in protein biomacromolecule chemistry remain rare. The development of efficient, complete, chemo- & regio-selective methods, applied in benign aqueous systems to redesign the structure and function of proteinsin vitroandin vivowill be presented[1-10].
(ii) ‘Synthetic Biologics’, Synthetic Biology and its application[10-20]: drug delivery; selective protein degradation; picomolar inhibitors of bacterial and viral interactions; gene delivery vehicles; probes of in vivo function; non-invasive pre-symptopmatic disease diagnosis; targeted high intensity radioprobes; novel glycoproteomic strategies; probes of mechanistic glycobiology; designed glycoconjugate vaccines and chemical ‘switching’ ofin vivo glycobiology.
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