In this study Langmuir monolayer technique is involved in monitoring interactions of selected local anaesthetics (LAs) - lidocaine, prilocaine, mepivacaine and ropivacaine - with monolayers mimicking erythrocyte, mitochondria and nerve membranes.
In the first step of investigations, the interactions of LAs with pure monolayers of 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC), cholesterol (Chol), sphingomyelin (SM) and cardiolipin (CL) were examined. It was found that the presence of LAs in the subphase increases the area occupied by lipid molecules. From all the drugs studied, the highest expansion was observed for ropivacaine. Among the investigated membrane lipids, the strongest effect of LAs was noticed for cardiolipin.
In the next step, the effect of LAs with model erythrocyte (POPC:SM:Chol=1:0.9:1.3), nerve cell (POPC:SM:Chol=1:0.9:2.6) and mitochondria (POPC:CL:Chol=1:0.35:0.1) membrane was studied. Fluidizing effect on model membranes was observed in all the cases. The strongest influence was noticed for nerve cell membrane.
The Langmuir monolayer investigations have been complemented with other techniques, i.e. PM-IRRAS and BAM.
mildner@chemia.uj.edu.pl
