CTAB (cetyltrimethylammonium bromide) and Septonex (carbethoxypendecinium bromide) are cationic surfactants known for harmful effects on different cell types (bacteria, fungi, mammal cells, etc.). Colloidal complexes of CTAB or Septonex with oppositely charged hyaluronic acid (HyA) were prepared primarily by their electrostatic interactions with the aim of their potential application in drug delivery. HyA seems to be promising biomaterial of near future due to its broad range of biological functions - combination of its biocompatibility and biodegradability properties and its susceptibility of chemical modifications. HyA can serve as a biocompatible carrier of a delivery system in which the surfactant serves as a solubilizer for hydrophobic active substance (drug). Our data show, that HyA complexes with CTAB or Septonex reduce (in different rate according to used antiseptic and HyA concentrations) cytotoxicity of both antiseptics in all concentrations tested. In addition, a significant role of FBS (important supplement of cell culture medium) in cell recovery under the stress conditions like CTAB or Septonex presence was observed. Taken together, HyA could be a useful tool for modulation of CTAB or Septonex effects on cells at diverse levels.
pekar@fch.vutbr.cz
