A large number of toxicological and medical papers have suggested the effect of nanoparticles using methods that lack biocompatible nanoparticle-dispersion protocols. Nano-assessment requires careful consideration of biological molecular adsorption on the nanoparticle. However, there is no uniform standard for safety evaluation. The relationship between nanoparticle-physicochemical properties and exact effects remains unclear. Recently, due to the progress of nanoparticle researches, we are coming to understand that the nanoparticle surface is an essential factor for decisions of cellular recognition and response. Hence, it is necessary to broaden our understanding of the biological molecular adsorption mechanism, which can interact with nanoparticles. Biological molecules can adsorb on nanoparticle surfaces with physical forces. However, the mechanism by which this occurs has not yet been clarified. The present study demonstrates physisorption of phospholipids on colloidal nanoparticles using phospholipids with different hydrophilic groups and lengths of saturated fatty acid hydrocarbon chains, and evaluates cellular uptake of phospholipid-adsorbed nanoparticles.
Acknowledgements:
The auther thanks Mr. Retsu Taniguchi in Tokyo University of Science for expert technical assistance in nanoparticle analyses.
