Biofilms are diverse biological structures. Most biofilms are associated with pathogenicity or adverse effects. Affecting the "life" in the biofilm is a challenge with no full scientific answer yet. The common means of affecting biofilm nowadays is via antimicrobial agents. However, they bear many ecological hazardous and side effects which render them as an ultimate solution for biofilms. One mode by which bacteria adapt to the biofilm environment is quorum sensing. This cell-cell communication regulates diverse sets of; physiological changes, virulence properties, adhesion modes, and protection modes, allowing the microbes to persist in the biofilm under stressed environmental changes. We have synthesized a line of molecules (Thiazolidinediones, TZD) which have been shown to affect biofilms. The TZD affect the quorum sensing cascade which further reflects in phenotypic properties as: biofilm formation, swarming, extra cellular polysaccharides production, altered cell hydrophobicity. A structure function analysis was established indicating that the side chain of the molecule is of importance to its biological effects. To further maximize the potential anti biofilm effect of the TZD we have developed a pharmaceutical technology of sustained release drug delivery system, in which the TZD is gradually release in control kinetics from the polymeric matrix. Anti biofilm, anti quorum sensing agents incorporated in a polymeric matrix may be a future drug/drug delivery systems affecting biofilm in high efficacy with less environment hazards and side effects.
